Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 783-792

Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Éboli, Lígia Patrícia de Carvalho BatistaSalzedas Netto, Alcides AugustoAzevedo, Ramiro Antero deLanzoni, Valéria PereiraPaula, Tatiana Sugayama deGoldenberg, AlbertoGonzalez, Adriano Miziara

PURPOSE:To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation.METHODS:Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) .RESULTS:Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed.CONCLUSION:King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.(AU)

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