Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil
Silva Filho, Osmar Ferreira daArgôlo Neto, Napoleão MartinsCarvalho, Maria Acelina Martins deCarvalho, Yulla Klinger deDiniz, Anaemilia das NevesMoura, Laécio da SilvaAmbrósio, Carlos EduardoMonteiro, Janaína MunueraAlmeida, Hatawa Melo deMiglino, Maria AngélicaAlves, Jacyara de Jesus Rosa PereiraMacedo, Kássio VieiraRocha, Andressa Rego daFeitosa, Matheus Levi TajraAlves, Flávio Ribeiro
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.(AU)
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