Nitric oxide levels in cochlear fluids of guinea pig
Yonamine, Fernando KaoruOnishi, Ektor TsuneoMouro, Margareth GoriHiga, Elisa Mieko SuemitsuCruz, Oswaldo Laércio Mendonça
PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.(AU)
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